孙雪松 研究员

发布者:发布时间:2024-06-04浏览次数:12

个人简介:

孙雪松,研究员,博士研究生导师,生化系副系主任。分别于1999年和2002年于南开大学生科院获得学士和硕士学位,2006年在香港大学化学系获得博士学位,2008年起在暨南大学历任副研究员和研究员。团队长期致力于细菌毒力和耐药,及其与宿主互作相关的机制研究,发现了多个对多耐药菌有效的抗菌药物并阐明了作用机制。自主研发了细菌蛋白组学定量新方法,建立多维度细菌蛋白组学数据库。2011年入选广州市“珠江科技新星”计划和暨南大学“杰出青年学者”计划。发表SCI论文发表50篇,其中第一作者及通讯作者40余篇(包括Cell Report, J Hazard Mater, mSystems, J Biol Chem,J Proteome Res等),单篇最高引用率达300余次,授权专利7项。近年主持国家重点研发计划子课题1项,国家科技部”973“子课题1项,国家自然科学基金4项,广东省自然科学基金4项,广州市科技计划2项,作为Front Cell Infect Microbiol 等杂志的编委、国家自然科学基金、广东省自然基金委等评委。

 

职称/职务:研究员,生物化学与分子生物学系 副主任

 

学习经历:

1995.09-1999.07  南开大学 生命科学院微生物系 本科

1999.09-2002.07  南开大学 生命科学院微生物系/中科院微生物所 硕士研究生

2003.10-2006.12  香港大学 化学系 博士研究生

 

工作经历:

2007.01-2008.02  香港大学 化学系 博士后

2008.03-2011.12  暨南大学 生命科学技术学院生物化学与分子生物学系 副研究员

2012.01-至今    暨南大学 生命科学技术学院生物化学与分子生物学系 研究员

 

研究方向:细菌毒力、耐药、与宿主互作相关的机制研究

 

主要论文(近十年以通讯作者发表的主要论文):

1. Zhong  T, Wu H, Hu J, Liu Y, Zheng Y, Li N, Sun Z, Yin XF, He QY, Sun X. Two  synonymous single-nucleotide polymorphisms promoting fluoroquinolone  resistance of Escherichia coli in the environment. J Hazard Mater. 2024,  469,133849. doi: 10.1016/j.jhazmat.2024.133849.

2. Fang  Z, Zhao X, Zhang Z, Wu J, Cheng J, Lei D, Li N, Ge R, He QY, Sun X.  Unveiling a novel mechanism for competitive advantage of  ciprofloxacin-resistant bacteria in the environment through bacterial  membrane vesicles. J Hazard Mater. 2024, 466, 133453. doi:  10.1016/j.jhazmat.2024.133453.

3. Fang  Z, Lai F, Cao K, Zhang Z, Cao L, Liu S, Duan Y, Yin X, Ge R, He QY, Sun  X. Potential Role of Lysine Acetylation in Antibiotic Resistance of  Escherichia coli. mSystems. 2022, 7(6), e0064922. doi:  10.1128/msystems.00649-22.

4. Cao  K, Zhang T, Li N, Yang XY, Ding J, He QY, Sun X. Identification and  Tetramer Structure of Hemin-Binding Protein SPD_0310 Linked to Iron  Homeostasis and Virulence of Streptococcus pneumoniae. mSystems. 2022,  7(3), e0022122. doi: 10.1128/msystems.00221-22.

5. Zheng  YD, Pan Y, He K, Li N, Yang D, Du GF, Ge R, He QY, Sun X. SPD_1495  Contributes to Capsular Polysaccharide Synthesis and Virulence in  Streptococcus pneumoniae. mSystems. 2020, 5(1), e00025-20. doi:  10.1128/mSystems.00025-20.

6. Fang  ZY, Zhang ZY, Zheng YD, Lei D, Zhuang J, Li N, He QY, Sun X.  Repurposing cinacalcet suppresses multidrug-resistant Staphylococcus  aureus by disruption of cell membrane and inhibits biofilm by targeting  IcaR. J Antimicrob Chemother. 2024, 79(4), 903-917. doi:  10.1093/jac/dkae051.

7. Lei  D, Cao L, Zhong T, He QY, Sun X. Residue Lys219 of CpxR is critical in  the regulation of the antibiotic resistance of Escherichia coli. J  Antimicrob Chemother. 2023, 78(8), 1859-1870. doi: 10.1093/jac/dkad171.

8. Cao  K, Li N, Wang H, Cao X, He J, Zhang B, He QY, Zhang G, Sun X. Two  zinc-binding domains in the transporter AdcA from Streptococcus pyogenes  facilitate high-affinity binding and fast transport of zinc. J Biol  Chem. 2018, 293(16), 6075-6089. doi: 10.1074/jbc.M117.818997.

9. Zheng  YD, Zhong T, Wu H, Li N, Fang Z, Cao L, Yin XF, He QY, Ge R, Sun X.  Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria  by Reducing ATP Production and Targeting the CTP Synthase PyrG.  Microbiol Spectr. 2022, 10(3), e0088422. doi: 10.1128/spectrum.00884-22.

10. Liu  J, Wei Q, Wang Z, Sun X, He QY. Proteomic Study of the Adaptive  Mechanism of Ciprofloxacin-Resistant Staphylococcus aureus to the Host  Environment. J Proteome Res. 2022, 21(6), 1537-1547. doi:  10.1021/acs.jproteome.2c00140.

11. Dong  Y, Miao X, Zheng YD, Liu J, He QY, Ge R, Sun X. Ciprofloxacin-Resistant  Staphylococcus aureus Displays Enhanced Resistance and Virulence in  Iron-Restricted Conditions. J Proteome Res. 2021, 20(5), 2839-2850. doi:  10.1021/acs.jproteome.1c00077.

12. Du  GF, Yin XF, Yang DH, He QY, Sun X. Proteomic Investigation of the  Antibacterial Mechanism of trans-Cinnamaldehyde against Escherichia  coli. J Proteome Res. 2021, 20(5), 2319-2328. doi:  10.1021/acs.jproteome.0c00847.

13. Yang  DH, Liu S, Cao L, Zheng YD, Huang JF, Ge R, He QY, Sun X. Quantitative  secretome analysis of polymyxin B resistance in Escherichia coli.  Biochem Biophys Res Commun. 2020, 530(1), 307-313. doi:  10.1016/j.bbrc.2020.07.010.

14. Du  GF, Le YJ, Sun X, Yang XY, He QY. Proteomic investigation into the  action mechanism of berberine against Streptococcus pyogenes. J  Proteomics. 2020, 215, 103666. doi: 10.1016/j.jprot.2020.103666.

15. Du  GF, Zheng YD, Chen J, He QY, Sun X. Novel Mechanistic Insights into  Bacterial Fluoroquinolone Resistance. J Proteome Res. 2019, 18(11),  3955-3966. doi: 10.1021/acs.jproteome.9b00410.

16. Shi  T, Wei Q, Wang Z, Zhang G, Sun X, He QY. Photocatalytic Protein Damage  by Silver Nanoparticles Circumvents Bacterial Stress Response and  Multidrug Resistance. mSphere. 2019, 4(3), e00175-19. doi:  10.1128/mSphere.00175-19.

17. Han  J, Yi S, Zhao X, Zheng Y, Yang D, Du G, Yang XY, He QY, Sun X. Improved  SILAC method for double labeling of bacterial proteome. J Proteomics.  2019, 194, 89-98. doi: 10.1016/j.jprot.2018.12.011.

18. Cao  K, Lai F, Zhao XL, Wei QX, Miao XY, Ge R, He QY, Sun X. The mechanism  of iron-compensation for manganese deficiency of Streptococcus  pneumoniae. J Proteomics. 2018, 184, 62-70. doi:  10.1016/j.jprot.2018.06.004.

19. Miao  X, He J, Zhang L, Zhao X, Ge R, He QY, Sun X. A Novel Iron Transporter  SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence  Properties. Front Microbiol. 2018, 9, 1624. doi:  10.3389/fmicb.2018.01624.

20. Cao  K, Zhang J, Miao XY, Wei QX, Zhao XL, He QY, Sun X. Evolution and  molecular mechanism of PitAs in iron transport of Streptococcus species.  J Inorg Biochem. 2018, 182, 113-123. doi:  10.1016/j.jinorgbio.2018.02.006.

21. Liu  YT, Pan Y, Lai F, Yin XF, Ge R, He QY, Sun X. Comprehensive analysis of  the lysine acetylome and its potential regulatory roles in the  virulence of Streptococcus pneumoniae. J Proteomics. 2018, 176, 46-55.  doi: 10.1016/j.jprot.2018.01.014.

22. Yang  XY, He K, Du G, Wu X, Yu G, Pan Y, Zhang G, Sun X, He QY. Integrated  Translatomics with Proteomics to Identify Novel Iron-Transporting  Proteins in Streptococcus pneumoniae. Front Microbiol. 2016, 7, 78. doi:  10.3389/fmicb.2016.00078.

23. Guo  Z, Han J, Yang XY, Cao K, He K, Du G, Zeng G, Zhang L, Yu G, Sun Z, He  QY, Sun X. Proteomic analysis of the copper resistance of Streptococcus  pneumoniae. Metallomics. 2015, 7(3), 448-54. doi: 10.1039/c4mt00276h.

24. Yang  XY, Zhang L, Liu J, Li N, Yu G, Cao K, Han J, Zeng G, Pan Y, Sun X, He  QY. Proteomic analysis on the antibacterial activity of a Ru(II) complex  against Streptococcus pneumoniae. J Proteomics. 2015, 115, 107-116.  doi: 10.1016/j.jprot.2014.11.018.

25. Wu  X, Xu L, Gu W, Xu Q, He QY, Sun X, Zhang G. Iterative genome correction  largely improves proteomic analysis of nonmodel organisms. J Proteome  Res. 2014, 13(6), 2724-34. doi: 10.1021/pr500369b.

26. Li  N, Yang XY, Guo Z, Zhang J, Cao K, Han J, Zhang G, Liu L, Sun X, He QY.  Varied metal-binding properties of lipoprotein PsaA in Streptococcus  pneumoniae. J Biol Inorg Chem. 2014, 9(6), 829-38. doi:  10.1007/s00775-014-1114-9.

27. Wang  N, Zhang J, Zhang L, Yang XY, Li N, Yu G, Han J, Cao K, Guo Z, Sun X,  He QY. Proteomic analysis of putative heme-binding proteins in  Streptococcus pyogenes. Metallomics. 2014, 6(8), 1451-9. doi:  10.1039/c4mt00027g.

28. Ge  R, Sun X. Iron acquisition and regulation systems in Streptococcus  species. Metallomics. 2014, 6(5), 996-1003. doi: 10.1039/c4mt00011k.

 

承担课题:

1. 蛋白质修饰组的高纬度鉴定及功能网络(2017YFA0505100)。国家重点研发计划子课题,80万,2017.7-2022.6

2. 巴豆酰转移酶SPD_0839对肺炎链球菌铁摄取和毒力调控机制的研究 (22377035)。国家自然基金-面上项目,50万,2024.1-2026.12.

3. 肺炎链球菌SPD_0310蛋白介导的转铁机制及其在细菌毒力方面的功能研究(21977037)。国家自然基金-面上项目,66万,2020.1-2023.12

4. 肺炎链球菌锌离子摄取关键脂蛋白AdcA和AdcAll金属结合特性的研究(21571082)。国家自然基金-面上项目,72万,2016.1-2019.12

5. 巴多索隆抗菌效果的评价和作用机制的研究 (2023A1515011750)。广东省自然科学基金-面上项目,10万,2023.1-2025.12

6. 盐酸西那卡塞作为新型抗菌剂的抗菌效果的评价和靶点机制研究 (2022A1515010674)。广东省自然科学基金-面上项目,10万,2022.1-2024.12

7. 同位素标记技术结合MALDI-TOF MS 检测食品和相关基质中的细菌。国际项目,25万,2021.12-2026.12

8. 耐药细菌动态磷酸化蛋白质组的研究(201607010228)。广州市科技计划,20万,2016.4-2019.3

9. 肺炎链球菌新型转铁蛋白UgpB的发现和功能表征(c15140500000642)。广东省自然基金-面上项目,10万,2015-8-2018.8

10. 一种临床耐药菌检测试剂盒的开发。横向课题,10万,2016.1-2018.12

 

授权专利:

1. 巴多索隆在制备抗菌药物中的应用; CN202210593631.3.

2. Evacetrapib在制备抗菌药物中的应用; CN202210593633.2.

3. 克唑替尼在制备抗革兰氏阳性细菌药物中的应用; CN201811618190.8.

4. SPD_0310蛋白作为靶点在制备用于防治肺炎链球菌感染的药物中的应用; CN201810922798.3.

5. 一种微生物生长图像检测微室微流控系统; CN201810312529.5.

6. 一种利用精氨酸标记细菌的SILAC培养基及其制备方法与应用; CN201610391015.4.

7. 一种标记细菌的SILAC培养基及其制备方法与应用; CN201610390913.8.

 

讲授课程:

《生物化学》本科必修课主讲

《生物化学实验》本科必修课主讲

《功能蛋白质研究》研究生选修课

 

荣誉奖励:广州市首批珠江科技新星

 

电子邮箱:tsunxs@jnu.edu.cn

办公地址:暨南大学(石牌校区)第二理工楼918